Characterization of an enzyme from Rhizoctonia praticola which polymerizes phenolic compounds.

@article{Bollag1979CharacterizationOA,
  title={Characterization of an enzyme from Rhizoctonia praticola which polymerizes phenolic compounds.},
  author={Jean Marc Bollag and Roy D. Sjoblad and Shi Ying Liu},
  journal={Canadian journal of microbiology},
  year={1979},
  volume={25 2},
  pages={
          229-33
        }
}
An extracellular phenol oxidase from the fungus Rhizoctonia praticola which polymerizes various xenobiotic phenols was isolated and characterized. The enzyme was purified by DEAE-cellulose and Sephadex G-200 chromatography followed by preparative polyacrylamide gel electrophoresis. Atomic absorption and EPR spectroscopy indicated the presence of copper, and SDS gel electrophoresis revealed a molecular weight of 78,000. With 2,6-dimethoxyphenol as substrate, the enzyme showed a pH optimum of 6.7… CONTINUE READING
BETA