Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins.

@article{Dionne2005CharacterizationOA,
  title={Characterization of an archaeal family 4 uracil DNA glycosylase and its interaction with PCNA and chromatin proteins.},
  author={Isabelle J Dionne and Stephen D Bell},
  journal={The Biochemical journal},
  year={2005},
  volume={387 Pt 3},
  pages={859-63}
}
We describe the characterization of a family 4 UDG1 (uracil DNA glycosylase) from the crenarchaeote Sulfolobus solfataricus. UDG1 is found to have a marked preference for substrates containing a G:U base pair over either A:U or single-stranded uracil-containing DNA substrates. UDG1 is found to interact with the sliding clamp PCNA (proliferating cell nuclear antigen), and does so by a conserved motif in the C-terminus of the protein. S. solfataricus has a heterotrimeric PCNA, and only one of the… CONTINUE READING