Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp

  title={Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp},
  author={S. Gurusiddaiah and David M. Weller and A. Sarkar and Robert J. Cook},
  journal={Antimicrobial Agents and Chemotherapy},
  pages={488 - 495}
The production, isolation, and characterization of an antibiotic substance from cultures of Pseudomonas fluorescens 2-79 (NRRL B-15132) is described. P. fluorescens 2-79 originally was isolated from the roots of wheat and is suppressive to the wheat root disease take-all caused by Gaeumannomyces graminis var. tritici. The antibiotic was isolated from potato glucose broth cultures of strain 2-79 by solvent extraction. It was purified by silica gel column chromatography and was a greenish yellow… 
Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici
Results indicate that sequences required for phenazine production were present in the cloned DNA and support the importance of the phenazine antibiotic in disease suppression in the rhizosphere.
Production, purification, and characterization of antifungal metabolite from Pseudomonas aeruginosa SD12, a new strain obtained from tannery waste polluted soil.
The strain was used as a biocontrol agent against root rot and wilt disease of pyrethrum caused by Rhizoctonia solani and showed broad-spectrum antagonistic activity towards a range of phytopathogenic fungi, which is the first report of its kind.
Chromatographic Detection of Antibacterial Antibiotics from Pseudomonas fluorescens (TNAU PF1) and Their Effect on Bacterial Wilt Pathogen Ralstonia solanacearum (KK2)
The ability of crude antibiotics in inhibiting Ralstonia colonies was higher than the individual antibiotics (2,4-diacetylphloroglucinol and Phenazine) under in vitro.
Defined media for optimal pyoverdine production by Pseudomonas fluorescens 2-79
A Plackett-Burman design was used to identity eight amino acids, out of the 19 present in casamino acids, that were responsible for the increased pyoverdine production: methionine, valine, isoleucine, tyrosine, proline, phenylalanine, glutamic acid, and glycine.
Production of the Antibiotic Phenazine-1-Carboxylic Acid by Fluorescent Pseudomonas Species in the Rhizosphere of Wheat
In steamed and natural soils, roots from which the antibiotic was recovered had significantly less disease than roots with no antibiotic, indicating that suppression of take-all is related directly to the presence of the antibiotic in the rhizosphere.
Isolation and Identification of Antifungal 7 V-Butylbenzenesulphonamide Produced by Pseudomonas sp . AB 2
An antifungal bacterial strain, isolated from a greenhouse soil sample, inhibits growth of micro flora nearby. It was selected for further studies of bacterial antifungal properties. This isolate was
Revised structure for the phenazine antibiotic from Pseudomonas fluorescens 2-79 (NRRL B-15132)
Data suggest that phenazine-1-carboxylic acid is probably not an effective biological control agent for phytopathogens in environments with a pH greater than 7.0, and that an authentic antibiotic obtained from D. M. Weller was indistinguishable in all of its measured physicochemical and biological properties.


Suppression of take-all of wheat by seed treatments with fluorescent pseudomonads.
Howell and three, or possibly more consecutive crops of wheat after alfalfa or Stipanovic (7,8) demonstrated that strains of Pseudomonas potatoes, to help eliminate soilborne pathogens of those crops.
A method is described for the purification of two phenazine compounds from cultures of a strain of Pseudomonas aureofaciens. The chemical properties of the compounds are described and the
Simultaneous production of three phenazine pigments by Pseudomonas aeruginosa Mac 436.
Pseudomonas aeruginosa Mac 436 was found to produce simultaneously three phenazine pigments identified as pyocyanine, phenazine-1-carboxylic acid, and oxychlororaphine, and satisfactory yields of all three pigments were obtained.
Pattern of phenazine pigment production by a strain of Pseudomonas aeruginosa
An atypical strain of Pseudomonas aeruginosa capable of synthesizing three phenazine pigments was isolated, setting this strain apart from previously described chlororaphin producers.
1H NMR spectra of substituted phenazines
The 1H NMR Spectra of 28 substituted phenazines have been measured and analysed with computer assistance. Substituent increments of chemical shifts were calculated for methoxy, carbomethoxy and
Pantomycin: a new antimicrobial antibiotic.
Pantomycin is a new antibiotic isolated from a group of antibiotics produced in fermentation cultures of Streptomyces hygroscopicus. Pantomycin is a triad composed of carbohydrate, lipid, and polyp...
Two simple media for the demonstration of pyocyanin and fluorescin.
Abstract Two simple media for the enhancement of pigment production by certain organisms of the Pseudomonas genus are described. The results of comparative studies employing these media, certain