Characterization of an anti-FLAG antibody binding protein in V. cholerae.

  title={Characterization of an anti-FLAG antibody binding protein in V. cholerae.},
  author={Jung-Ho Shin and M. Lanz and M. Smolka and Tobias D{\"o}rr},
  journal={Biochemical and biophysical research communications},
  • Jung-Ho Shin, M. Lanz, +1 author Tobias Dörr
  • Published 2020
  • Medicine, Biology
  • Biochemical and biophysical research communications
  • FLAG-tags are commonly used for protein abundance measurements and for identification of protein-protein interactions in living cells. We have observed that the cholera pathogen Vibrio cholerae encodes a FLAG-antibody-reactive protein and identified this protein as an outer membrane porin, Porin4, which contains a sequence very similar to the 3xFLAG epitope tag. We have demonstrated the binding affinity of the conserved peptide sequence (called Porin 4 tag) in Porin4 against monoclonal anti… CONTINUE READING

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    Overview of Affinity Tags for Protein Purification
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