Characterization of an Endoglucanase from Pseudomonas fluorescens subsp. cellulosa Produced in Escherichia coli and Regulation of the Expression of Its Cloned Gene.

@article{Lejeune1988CharacterizationOA,
  title={Characterization of an Endoglucanase from Pseudomonas fluorescens subsp. cellulosa Produced in Escherichia coli and Regulation of the Expression of Its Cloned Gene.},
  author={A. G. Henri Lejeune and Sophie Courtois and Charles Colson},
  journal={Applied and environmental microbiology},
  year={1988},
  volume={54 2},
  pages={302-8}
}
Several enzymatic properties of an endoglucanase produced in Escherichia coli by a gene from Pseudomonas fluorescens subsp. cellulosa were investigated. Gel filtration revealed a single peak of M(r) 36,000 with endoglucanase activity. The pH optimum of the enzyme was 7.0. Carboxymethyl cellulose and barley beta-glucan (mixed beta-1,3 and 1,4 linkages) were good substrates, but not laminarin (beta-1,3 linkages), amylose, filter paper, microcrystalline cellulose (Avicel), or cellotriose. The mode… CONTINUE READING