Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles

  title={Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles},
  author={S S Yang and Nelson A. Wivel},
  journal={Journal of Virology},
  pages={712 - 720}
An RNA-dependent DNA polymerase associated with intracisternal A particles has been characterized. The enzyme required Mg2+ or Mn2+, dithiothreitol and the presence of all four deoxyribonucleoside triphosphates for the expression of maximal activity. Sensitivity of the endogenous RNA-dependent DNA polymerase activity to a low concentration of pancreatic ribonuclease in the presence of a high concentration of NaCl suggested that the enzyme might be utilizing the A particle endogenous RNA as… 
Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles
Observations strongly indicate that the endogenous A-particle RNA serves as the template for the DNA polymerase.
Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells
The RNA isolated from purified A particles is generally of low molecular weight but contains small amount of 70S and 35S components, which suggest that A-type particles may be related to C-type oncornaviruses.
Properties of an intracisternal A-particle-associated endonuclease activity which is stimulated by ATP
An endonuclease activity associated with purified proteinase K-treated intracisternal A-particles was identified and characterized and was greatly stimulated by ATP, ADP, and dATP, whereas AMP appeared to produce a slight inhibition and GTP had no effect on the activity.
Purification and characterization of DNA polymerases from the plasmacytoma MOPC 104E and Abelson murine leukemia viruses.
RNA-dependent DNA polymerases of intracisternal A particles from the mouse plasma cell tumor MOPC 104E and of Abelson murine leukemia virus (A-MuLV) were isolated from particle preparations by
Intracisternal A particles from FLOPC-1 BALB/c myeloma: presence of high-molecular-weight RNA and RNA-dependent DNA polymerase
Intracisternal A particles from the FLOPC-1 line of BALB/c myeloma have been shown to contain high-molecular-weight RNA (60 to 70S) that is sensitive to RNase, alkali degradation, and heat but
RNA-directed DNA polymerase from particles released by normal goose cells
Goose cells producing DNA polymerase-containing particles did not contain reticuloendotheliosis virus-related nucleotide sequences in their DNA and the particles were not infectious and did not have endogenous DNA synthesis.
Properties of Intracytoplasmic A Particles Isolated from Oncornavirus-Producing Human Cells
A particles isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors ofason-pfizers-like on cornavirus but represent an independent oncornvirus.


A novel DNA polymerase activity found in association with intracisternal A-type particles.
  • S. H. Wilson, E. Kuff
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1972
A DNA polymerase activity that promotes the synthesis of poly(dT) has been found in association with intracisternal A-type particles isolated from several mouse tumors, and it is found to be optimal at high salt concentration, prefers magnesium over manganese, and is stimulated by poly(rA.
Analysis of High-Molecular-Weight Ribonucleic Acid Associated with Intracisternal A Particles
There are probably some taxonomic relationships between intracisternal A particles and oncogenic RNA viruses, and this HMW RNA is sensitive to ribonuclease digestion and alkali treatment but is resistant to Pronase treatment.
RNA-dependent DNA polymerase activity in five RNA viruses: divalent cation requirements.
A soluble RNA-dependent DNA polymerase activity has been released from murine leukemia particles in the presence of manganese and high detergent concentrations.
Mechanism of carcinogenesis by RNA tumor viruses. I. An RNA-dependent DNA polymerase in murine sarcoma viruses.
A highly active and stable DNA polymerase was found in purified preparations of two murine sarcoma viruses, suggesting that the template is RNA and the product is DNA.
Purification, Characterization, and Comparison of the DNA Polymerases from Two Primate RNA Tumor Viruses
The results show that more similarity exists between the DNA polymerases from viruses of the same type (type-C), than between the polymerasesfrom viruses of different types but from closely related species.
The extraction of intracisternal A-particles from a mouse plasma-cell tumor.
Evidence is presented that 40 to 50% of the total RNA in the isolated fractions was contributed by other cytoplasmic components and the remainder may have represented RNA intrinsic to the A-particles themselves; however, further studies are required to establish this point.