Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles
@article{Yang1974CharacterizationOA, title={Characterization of an Endogenous RNA-Dependent DNA Polymerase Associated with Murine Intracisternal A Particles}, author={S S Yang and Nelson A. Wivel}, journal={Journal of Virology}, year={1974}, volume={13}, pages={712 - 720} }
An RNA-dependent DNA polymerase associated with intracisternal A particles has been characterized. The enzyme required Mg2+ or Mn2+, dithiothreitol and the presence of all four deoxyribonucleoside triphosphates for the expression of maximal activity. Sensitivity of the endogenous RNA-dependent DNA polymerase activity to a low concentration of pancreatic ribonuclease in the presence of a high concentration of NaCl suggested that the enzyme might be utilizing the A particle endogenous RNA as…
55 Citations
Characterization of an RNA-directed DNA polymerase found in association with murine intracytoplasmic A-particles
- BiologyJournal of virology
- 1977
Observations strongly indicate that the endogenous A-particle RNA serves as the template for the DNA polymerase.
Characterization of DNA polymerase and RNA associated with A-type particles from murine myeloma cells
- BiologyJournal of virology
- 1975
The RNA isolated from purified A particles is generally of low molecular weight but contains small amount of 70S and 35S components, which suggest that A-type particles may be related to C-type oncornaviruses.
Physicochemical analysis of the deoxyribonucleic acid product of murine intracisternal A particle RNA-directed DNA polymerase.
- BiologyBiochimica et biophysica acta
- 1976
Properties of an intracisternal A-particle-associated endonuclease activity which is stimulated by ATP
- Biology, ChemistryJournal of virology
- 1981
An endonuclease activity associated with purified proteinase K-treated intracisternal A-particles was identified and characterized and was greatly stimulated by ATP, ADP, and dATP, whereas AMP appeared to produce a slight inhibition and GTP had no effect on the activity.
Polyadenylation of 35S RNA in vitro by a cytoplasmic particulate fraction from mouse myeloma.
- BiologyBiochemical and biophysical research communications
- 1976
Purification and characterization of DNA polymerases from the plasmacytoma MOPC 104E and Abelson murine leukemia viruses.
- BiologyEuropean journal of biochemistry
- 1978
RNA-dependent DNA polymerases of intracisternal A particles from the mouse plasma cell tumor MOPC 104E and of Abelson murine leukemia virus (A-MuLV) were isolated from particle preparations by…
Intracisternal A particles from FLOPC-1 BALB/c myeloma: presence of high-molecular-weight RNA and RNA-dependent DNA polymerase
- BiologyJournal of virology
- 1976
Intracisternal A particles from the FLOPC-1 line of BALB/c myeloma have been shown to contain high-molecular-weight RNA (60 to 70S) that is sensitive to RNase, alkali degradation, and heat but…
RNA-directed DNA polymerase from particles released by normal goose cells
- BiologyJournal of virology
- 1979
Goose cells producing DNA polymerase-containing particles did not contain reticuloendotheliosis virus-related nucleotide sequences in their DNA and the particles were not infectious and did not have endogenous DNA synthesis.
Properties of Intracytoplasmic A Particles Isolated from Oncornavirus-Producing Human Cells
- BiologyJournal of virology
- 1974
A particles isolated from the cytoplasm of HEp-2, HeLa, and AO cells producing oncornavirus of Mason-Pfizer-like type contained neither homological RNA sequences nor common antigens, suggesting that A particles are not intracellular precursors ofason-pfizers-like on cornavirus but represent an independent oncornvirus.
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