Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer.

@article{Ferreira2015CharacterizationOA,
  title={Characterization of allele-specific expression of the X-linked gene MAO-A in trophectoderm cells of bovine embryos produced by somatic cell nuclear transfer.},
  author={Allice Rodrigues Ferreira and L F C Aguiar Filho and Regivaldo V Sousa and Raissa Saran Sartori and Maur{\'i}cio Machaim Franco},
  journal={Genetics and molecular research : GMR},
  year={2015},
  volume={14 4},
  pages={
          12128-36
        }
}
Somatic cell nuclear transfer (SCNT) may affect epigenetic mechanisms and alter the expression of genes related to embryo development and X chromosome inactivation (XCI). We characterized allele-specific expression of the X-linked gene monoamine oxidase type A (MAO-A) in the trophectoderm (TF) of embryos produced by SCNT. Total RNA was isolated from individual biopsies (N = 25), and the allele-specific expression assessed by reverse transcription-polymerase chain reaction-restriction fragment… CONTINUE READING

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