Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone

@article{Sim2011CharacterizationOA,
  title={Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone},
  author={Su-Jung Sim and Keun Sik Baik and Seong Chan Park and Han Na Choe and Chi Nam Seong and Tai-Sun Shin and Hee Chul Woo and Jeong-Yong Cho and Duwoon Kim},
  journal={Journal of Industrial Microbiology & Biotechnology},
  year={2011},
  volume={39},
  pages={585-593}
}
A metagenomic fosmid library was constructed using a genomic DNA mixture extracted from the gut microflora of abalone. The library gave an alginate lyase positive clone (AlyDW) harboring a 31.7-kbp insert. The AlyDW insert consisted of 22 open reading frames (ORFs). The deduced amino acid sequences of ORFs 11–13 were similar to those of known alginate lyase… CONTINUE READING