Characterization of adenylyl cyclase isoforms in rat peripheral pulmonary arteries.

@article{Jourdan2001CharacterizationOA,
  title={Characterization of adenylyl cyclase isoforms in rat peripheral pulmonary arteries.},
  author={Karen B. Jourdan and N. A. Mason and Lu Long and Peter Philips and Martin R. Wilkins and Nicholas W. Morrell},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={2001},
  volume={280 6},
  pages={
          L1359-69
        }
}
Activation of adenylyl cyclase (AC), of which there are 10 diversely regulated isoforms, is important in regulating pulmonary vascular tone and remodeling. Immunohistochemistry in rat lungs demonstrated that AC2, AC3, and AC5/6 predominated in vascular and bronchial smooth muscle. Isoforms 1, 4, 7, and 8 localized to the bronchial epithelium. Exposure of animals to hypoxia did not change the pattern of isoform expression. RT-PCR confirmed mRNA expression of AC2, AC3, AC5, and AC6 and… 
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Methods Citations
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39 Citations

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Citation Type

Coordinate Down-regulation of Adenylyl Cyclase Isoforms and the Stimulatory G Protein (Gs) in Intestinal Epithelial Cell Differentiation*
TLDR
It is shown that cell differentiation in a model intestinal epithelium leads to attenuation of cAMP production in response to G protein-coupled receptor and receptor-independent agonists, and down-regulation of AC isoforms and Gsα is an integral part of the intestinal epithelial differentiation program.
Hypoxia inhibits adenylyl cyclase catalytic activity in a porcine model of persistent pulmonary hypertension of the newborn.
TLDR
Downregulation of AC-mediated relaxation in hypoxic PA has implications for utility of Gαs-coupled receptor agonists in PPHN treatment and isometric myography, ATP content, phosphodiesterase activity, AC content, isoform expression, and catalytic activity are studied.
Functional changes in adenylyl cyclases and associated decreases in relaxation responses in mesenteric arteries from diabetic rats.
TLDR
The results suggest that AC-mediated relaxation is impaired in the STZ-diabetic rat mesenteric artery, perhaps reflecting a reduction in AC5/6 activity.
Role of β-Adrenoceptor/Adenylyl Cyclase System in Cardiac Hypertrophy
TLDR
The physiological aspects of β-adrenergic signaling pathways and their modification in the hypertrophied heart as well as their participation in the transition of cardiac hypertrophy to heart failure are discussed.
Regulation of Phosphodiesterase 3 in the Pulmonary Arteries During the Perinatal Period in Sheep
TLDR
PDE3 expression and activity in the PA dramatically increase after birth, with a concomitant decrease in steady-state cAMP, and relaxation to milrinone, a PDE3 inhibitor, was greater in thePA isolated from 1dSB and 100% O2 + iNO lambs.
International Union of Basic and Clinical Pharmacology. CI. Structures and Small Molecule Modulators of Mammalian Adenylyl Cyclases
TLDR
Understanding of the (patho)physiology of AC isoforms and the development of clinically useful drugs targeting ACs is still in its infancy, in contrast to the guanylyl cyclase field.
Molecular analysis of mammalian adenylyl cyclases and bacterial adenylyl cyclase toxins
TLDR
By the use of several techniques, this thesis provides a pharmacological profile of cardiac AC and an excellent starting point for the design of potent and selective inhibitors of AC toxin inhibitors.
Characterization of contractile function and expression of muscarinic receptors, G proteins and adenylate cyclase in cultured tracheal smooth muscle of Swine.
TLDR
It is suggested that cultured smooth muscle might be used as a model to study the regulation of contractile function of smooth muscle by various signal transduction pathways.
Dominant regulation of interendothelial cell gap formation by calcium-inhibited type 6 adenylyl cyclase
TLDR
Findings provide the first direct evidence that calcium inhibition of AC6 is essential for endothelial gap formation, which virtually abolished thrombin-induced gap formation in PMVECs.
Characterization of Contractile Function and Expression of Muscarinic Receptors, G Proteins and Adenylate Cyclase in Cultured Tracheal Smooth Muscle of Swine
TLDR
The results show that contractile function and the amounts of M3 receptors, G proteins and adenylyl cyclase were maintained for up to 5 days in culture, and suggest that cultured smooth muscle might be used as a model to study the regulation ofcontractile function of smooth muscle by various signal transduction pathways.
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References

SHOWING 1-10 OF 58 REFERENCES
Inhibition by Calcium of Mammalian Adenylyl Cyclases*
TLDR
The present results show that high and low affinity inhibition by Ca2+ can be clearly distinguished and that the inhibition occurs type-specifically in discrete adenylyl cyclases.
Prostacyclin production and mediation of adenylate cyclase activity in the pulmonary artery. Alterations after prolonged hypoxia in the rat.
TLDR
It is postulated that attenuation of the response of adenylate cyclase to prostacyclin may contribute to the structural changes and hypertension observed in the pulmonary vasculature of the rat with chronic hypoxia.
Inflammatory and contractile agents sensitize specific adenylyl cyclase isoforms in human airway smooth muscle.
TLDR
Chronic exposure of human ASM (HASM) to carbachol, serotonin, the thromboxane analogue U46619, or histamine induced little change or a small increase in isoproterenol-stimulated cyclic adenosine monophosphate (cAMP) formation, but significantly increased cAMP formation elicited by stimulation with forskolin.
Evidence for Gβγ-mediated Cross-talk in Primary Cultures of Lung Alveolar Cells
TLDR
Type II cells expressing endogenous AC II and IV regulate cAMP accumulation and AC activity in a manner consistent with conditional stimulation by βγ, which supports the overall hypothesis that conditional stimulation of AC by α2-adrenergic agonist integrates cross-talk between signal transduction systems in differentiated cells.
Mechanisms regulating cAMP-mediated growth of bovine neonatal pulmonary artery smooth muscle cells.
TLDR
It is indicated that protein kinase C stimulation of cAMP is a critical signal mediating proliferation of neonatal PASMCs that is absent in adult PAS MCs and therefore may contribute to the unique proproliferative phenotype of these neonatal cells.
Ca(2+)-inhibitable adenylyl cyclase modulates pulmonary artery endothelial cell cAMP content and barrier function.
TLDR
The results indicate that a type VI Ca(2+)-inhibitable adenylyl cyclase exists in pulmonary artery endothelial cells and is modulated by physiological changes in Ca2+i, and suggest the inverse relationship between [Ca2+]i and cAMP that is established by Ca( 2+)-Inhibitable AdenylyL cyclase plays a critical role in modulating pulmonary artery vascular cell permeability.
Forskolin derivatives with increased selectivity for cardiac adenylyl cyclase.
TLDR
The results suggest that a particular side-chain modification of forskolin enhanced the selectivity for the cardiac isoform stimulation and may be targeted to increase tissue selectivity in future drug therapy for beta-adrenergic regulation.
Phosphorylation and Inhibition of Type III Adenylyl Cyclase by Calmodulin-dependent Protein Kinase II in Vivo *
TLDR
It is reported that III-AC is phosphorylated in vivo when intracellular Ca2+ is increased and that phosphorylation is prevented by CaM-dependent kinase inhibitors.
Adenylyl cyclase isoforms and vasopressin enhancement of agonist-stimulated cAMP in vascular smooth muscle cells.
TLDR
The results indicate that AVP activates V1 receptors in VSMC to enhance agonist-stimulated cAMP formation by a Ca(2+)-calmodulin-dependent mechanism and suggest that type III adenylyl cyclase may provide a focal point in the VSMC for cross talk between constrictor and dilator pathways.
Molecular cloning and characterization of a Ca2+/calmodulin-insensitive adenylyl cyclase from rat brain.
TLDR
The existence of multiple adenylyl cyclase enzymes may provide for complex and distinct modes of biochemical regulation of cAMP levels in the brain.
...
1
2
3
4
5
...