Characterization of adeno-associated virus rep proteins in human cells by antibodies raised against rep expressed in Escherichia coli.

Abstract

The rep gene of the defective human parvovirus, adeno-associated virus, (AAV) mediates several trans-acting functions important to virus replication, transcription, and gene expression. At least four overlapping polypeptides are expressed from the rep gene. We have constructed a prokaryotic vector which expressed in Escherichia coli a region of AAV… (More)

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