Characterization of active-site residues of the NIa protease from tobacco vein mottling virus.

@article{Hwang2000CharacterizationOA,
  title={Characterization of active-site residues of the NIa protease from tobacco vein mottling virus.},
  author={Daye Hwang and Doo Hwan Kim and June Seung Lee and Byoung Heon Kang and Joo Seok Han and Won-Tae Kim and Byeong Doo Song and Kwan Yong Choi},
  journal={Molecules and cells},
  year={2000},
  volume={10 5},
  pages={505-11}
}
Nuclear inclusion a (NIa) protease of tobacco vein mottling virus is responsible for the processing of the viral polyprotein into functional proteins. In order to identify the active-site residues of the TVMV NIa protease, the putative active-site residues, His-46, Asp-81 and Cys-151, were mutated individually to generate H46R, H46A, D81E, D81N, C151S, and C151A, and their mutational effects on the proteolytic activities were examined. Proteolytic activity was completely abolished by the… CONTINUE READING