Characterization of acid-tolerant H/CO-utilizing methanogenic enrichment cultures from an acidic peat bog in New York State.

@article{Bruer2006CharacterizationOA,
  title={Characterization of acid-tolerant H/CO-utilizing methanogenic enrichment cultures from an acidic peat bog in New York State.},
  author={Suzanna L. Br{\"a}uer and Erika Yashiro and Norikiyo G Ueno and Joseph B. Yavitt and Stephen H. Zinder},
  journal={FEMS microbiology ecology},
  year={2006},
  volume={57 2},
  pages={206-16}
}
Two methanogenic cultures were enriched from acidic peat soil using a growth medium buffered to c. pH 5. One culture, 6A, was obtained from peat after incubation with H(2)/CO(2), whereas culture NTA was derived from a 10(-4) dilution of untreated peat into a modified medium. 16S rRNA gene clone libraries from each culture contained one methanogen and two bacterial sequences. The methanogen 16S rRNA gene sequences were 99% identical with each other and belonged to the novel "R-10/Fen cluster… CONTINUE READING
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