Characterization of acid-induced partially folded conformation resembling a molten globule state of polygalacturonase from a filamentous fungus Tetracoccosporium sp.

Abstract

Acid-induced unfolding of a Tetracoccosporium sp. polygalacturonase enzyme (PG) was studied by a comprehensive series of biophysical and biochemical techniques. At pH 1.0, PG acquires partially folded state, which reveals characteristics of molten globule (MG) state, i.e., reduction of defined tertiary structure with minimal changes in the secondary… (More)
DOI: 10.1007/s12010-009-8723-8

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