Characterization of absorption and scattering properties of small-volume biological samples using time-resolved spectroscopy.

@article{Liu1993CharacterizationOA,
  title={Characterization of absorption and scattering properties of small-volume biological samples using time-resolved spectroscopy.},
  author={Hui Liu and Motoatsu Miwa and Bertrand Beauvoit and Nan Wang and Britton Chance},
  journal={Analytical biochemistry},
  year={1993},
  volume={213 2},
  pages={378-85}
}
With time-resolved spectroscopy, we develop an experimental approach by sample substitution to measure the absorption (mu a) and reduced scattering (mu's) coefficients of small-volume biological samples. To investigate the method, small-volume control samples are substituted into a large-size host medium during increases in the absorber (or scatterer) concentration of the host. By characterizing the deviation of the spectra taken with and without the sample, we determine the matching points… CONTINUE READING