Characterization of a two-gene locus from Bartonella bacilliformis associated with the ability to invade human erythrocytes.

@article{Mitchell1995CharacterizationOA,
  title={Characterization of a two-gene locus from Bartonella bacilliformis associated with the ability to invade human erythrocytes.},
  author={Stephen J. Mitchell and Michael Minnick},
  journal={Infection and immunity},
  year={1995},
  volume={63 4},
  pages={1552-62}
}
Bartonella bacilliformis, the agent of human Oroya fever, invades erythrocytes and causes a severe hemolytic anemia. The ability of two minimally invasive strains of Escherichia coli (DH5 alpha and HB101) to invade human erythrocytes was enhanced 6- to 39-fold by transformation with pIAL1, a plasmid containing a 1,469-bp BamHI fragment from the B. bacilliformis chromosome. Invasiveness was confirmed by gentamicin protection and transmission electron microscopy. DNA hybridization analysis… CONTINUE READING