Characterization of a trehalose-degrading enzyme from the hyperthermophilic archaeon Sulfolobus acidocaldarius.

@article{Moon2016CharacterizationOA,
  title={Characterization of a trehalose-degrading enzyme from the hyperthermophilic archaeon Sulfolobus acidocaldarius.},
  author={Jeong Hyun Moon and Whiso Lee and Jihee Park and Kyoung-Hwa Choi and Jaeho Cha},
  journal={Journal of bioscience and bioengineering},
  year={2016},
  volume={122 1},
  pages={47-51}
}
We purified a cytosolic trehalase (TreH) from a thermoacidophilic archaeon Sulfolobus acidocaldarius. Enzyme activity in cell-free extracts indicated that trehalose degradation in the cell occurred via the hydrolytic activity of TreH, and not via TreP (phosphorolytic activity) or TreT (transfer activity). TreH was purified to near-homogeneity by DEAE anion-exchange chromatography, followed by size exclusion and HiTrap Q anion-exchange chromatography, and its molecular mass was estimated as 40… CONTINUE READING