Characterization of a transposon Tn916-generated mutant of Haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis.

@article{Gibson1997CharacterizationOA,
  title={Characterization of a transposon Tn916-generated mutant of Haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis.},
  author={Bradford W. Gibson and Anthony A. Campagnari and W. Melaugh and Nancy J. Phillips and Michael A. Apicella and Susan A Grass and Jia-wei Wang and K. L. Palmer and Robert S. Munson},
  journal={Journal of bacteriology},
  year={1997},
  volume={179 16},
  pages={5062-71}
}
To define the role of the surface lipooligosaccharide (LOS) of Haemophilus ducreyi in the pathogenesis of chancroid, Tn916 mutants of H. ducreyi 35000 defective in expression of the murine monoclonal antibody (MAb) 3F11 epitope on H. ducreyi LOS were identified by immunologic screening. One mutant, designated 1381, has an LOS which lacks the MAb 3F11 epitope and migrates with an increased mobility on sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The gene disrupted by the Tn916… CONTINUE READING
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