Characterization of a tobacco epoxide hydrolase gene induced during the resistance response to TMV.

@article{Guo1998CharacterizationOA,
  title={Characterization of a tobacco epoxide hydrolase gene induced during the resistance response to TMV.},
  author={Ailan Guo and Joerg Durner and Daniel F Klessig},
  journal={The Plant journal : for cell and molecular biology},
  year={1998},
  volume={15 5},
  pages={647-56}
}
A clone encoding a putative soluble epoxide hydrolase (EH-1), an enzyme which converts epoxides to diols, was isolated by differential screening of a cDNA library prepared from tobacco mosaic virus (TMV)-infected tobacco leaves. To confirm that EH-1 encodes an epoxide hydrolase, the recombinant EH-1 protein produced in bacteria was shown to have high epoxide hydrolase activity in vitro. Infection of resistant but not susceptible tobacco cultivars induced the accumulation of EH-1 transcripts in… CONTINUE READING