Characterization of a thermostable cyclodextrin glucanotransferase from Pyrococcus furiosus DSM3638

@article{Lee2007CharacterizationOA,
  title={Characterization of a thermostable cyclodextrin glucanotransferase from Pyrococcus furiosus DSM3638},
  author={Myoung-Hee Lee and Sung-jae Yang and Jung-Woo Kim and H Lee and J Kim and Kwan Hwa Park},
  journal={Extremophiles},
  year={2007},
  volume={11},
  pages={537-541}
}
A gene that encodes the enzyme Pyrococcus furiosus cyclodextrin glucanotransferase (PFCGT) was cloned in Escherichia coli. PFCGT was highly expressed in recombinant E. coli after compensation for codon usage bias using the pRARE plasmid. Purified PFCGT was extremely thermostable with an optimal temperature and pH of 95°C and 5.0, respectively, retaining 97% of its activity at 100°C. Incubation at 60°C for 20 min during the purification process led to a 1.5-fold increase in enzymatic activity. A… CONTINUE READING

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