Characterization of a thermosensitive Escherichia coli aspartyl-tRNA synthetase mutant.

Abstract

The Escherichia coli tls-1 strain carrying a mutated aspS gene (coding for aspartyl-tRNA synthetase), which causes a temperature-sensitive growth phenotype, was cloned by PCR, sequenced, and shown to contain a single mutation resulting in substitution by serine of the highly conserved proline 555, which is located in motif 3. When an aspS fragment spanning… (More)

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@article{Martn1997CharacterizationOA, title={Characterization of a thermosensitive Escherichia coli aspartyl-tRNA synthetase mutant.}, author={Francisco J. Mart{\'i}n and Gary J. Sharples and R. G. Lloyd and Sylvia Eiler and Dino Moras and Jean Gangloff and Gilbert Eriani}, journal={Journal of bacteriology}, year={1997}, volume={179 11}, pages={3691-6} }