Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release

@inproceedings{Pasotti2011CharacterizationOA,
  title={Characterization of a synthetic bacterial self-destruction device for programmed cell death and for recombinant proteins release},
  author={Lorenzo Pasotti and Susanna Zucca and Manuel Lupotto and Maria Gabriella Cusella De Angelis and Paolo Magni},
  booktitle={Journal of biological engineering},
  year={2011}
}
BACKGROUND Bacterial cell lysis is a widely studied mechanism that can be achieved through the intracellular expression of phage native lytic proteins. This mechanism can be exploited for programmed cell death and for gentle cell disruption to release recombinant proteins when in vivo secretion is not feasible. Several genetic parts for cell lysis have been developed and their quantitative characterization is an essential step to enable the engineering of synthetic lytic systems with… CONTINUE READING
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