Characterization of a sulfotransferase responsible for the 4-O-sulfation of terminal beta-N-acetyl-D-galactosamine on asparagine-linked oligosaccharides of glycoprotein hormones.

@article{Skelton1991CharacterizationOA,
  title={Characterization of a sulfotransferase responsible for the 4-O-sulfation of terminal beta-N-acetyl-D-galactosamine on asparagine-linked oligosaccharides of glycoprotein hormones.},
  author={Timothy P. Skelton and Lora V Hooper and Vibha Srivastava and Ole Hindsgaul and Jacques U. Baenziger},
  journal={The Journal of biological chemistry},
  year={1991},
  volume={266 26},
  pages={17142-50}
}
The Asn-linked oligosaccharides on the glycoprotein hormones lutropin (LH) and thyrotropin terminate with the sequence SO4-4GalNAc beta 1-4GlcNAc beta 1-2 Man alpha-. Using a chemically synthesized trisaccharide GalNAc beta 1-4GlcNAc beta 1-2Man alpha 1-O(CH2)8COOCH3 (GGnM-MCO), we have developed a sensitive assay for the sulfotransferase responsible for the 4-O-sulfation of the terminal beta-D-GalNAc. GGnM-MCO is incubated with a bovine pituitary membrane extract and [35S]3'-phosphoadenosine 5… CONTINUE READING
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Publications referenced by this paper.

, ed ) pp . 213 - 224 , American Chemical Society , Washington , D . C . Biophys

  • R. G. Schweiger
  • ACS Symposium Series 77 : Carbohydrate Sulfates
  • 1978

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