Characterization of a soluble class I alpha-mannosidase in human serum.

@article{Porwoll1999CharacterizationOA,
  title={Characterization of a soluble class I alpha-mannosidase in human serum.},
  author={Stefan Porwoll and Hendrik Fuchs and Rudolf Tauber},
  journal={FEBS letters},
  year={1999},
  volume={449 2-3},
  pages={175-8}
}
Class I alpha-mannosidases are thought to exist exclusively as integral membrane proteins that play intracellulary an essential role in the N-glycan biosynthesis. Using [3H]Man9GlcNAc2 as a substrate, we were able to identify a soluble alpha-mannosidase in human serum that trims the substrate Man9GlcNAc2 to Man(5-8)GlcNAc2 with Man6GlcNAc2 being the major… CONTINUE READING