Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system.

@article{Jung2003CharacterizationOA,
  title={Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system.},
  author={Ki-Hong Jung and Junghe Hur and Choong-Hwan Ryu and Youngju Choi and Y Chung and Akio Miyao and Hirohiko Hirochika and Gynheung An},
  journal={Plant & cell physiology},
  year={2003},
  volume={44 5},
  pages={463-72}
}
We have previously generated a large pool of T-DNA insertional lines in rice. In this study, we screened those T-DNA pools for rice mutants that had defective chlorophylls. Among the 1,995 lines examined in the T2 generation, 189 showed a chlorophyll-deficient phenotype that segregated as a single recessive locus. Among the mutants, 10 lines were beta-glucuronidase (GUS)-positive in the leaves. Line 9-07117 has a T-DNA insertion into the gene that is highly homologous to XANTHA-F in barley and… CONTINUE READING
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