Characterization of a rabbit polyclonal antibody against threonine-AMPylation.

  title={Characterization of a rabbit polyclonal antibody against threonine-AMPylation.},
  author={Y Hao and Trinette Chuang and Haydn L. Ball and P. N. Luong and Yan Li and Rub{\'e}n D. Flores-saaib and Kim Orth},
  journal={Journal of biotechnology},
  volume={151 3},
An antibody against the posttranslational modification AMPylation was produced using a peptide corresponding to human Rac1 switch I region with AMPylated threonine-35 residue as an antigen. The resulting rabbit antiserum was tested for its abilities to recognize AMPylated proteins by western blot and immunoprecipitation. The antiserum is highly specific for threonine-AMPylated proteins and weakly recognizes tyrosine-AMPylated proteins. Depletion of serum with modified protein abolished its… CONTINUE READING


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