Characterization of a protein-based adhesive elastomer secreted by the Australian frog Notaden bennetti.

  title={Characterization of a protein-based adhesive elastomer secreted by the Australian frog Notaden bennetti.},
  author={Lloyd D. Graham and Veronica Glattauer and Mickey G. Huson and Jane M. Maxwell and Robert B Knott and John William White and Paul R. Vaughan and Yong Y Peng and Michael J. Tyler and Jerome Anthony Werkmeister and John Alan Maurice Ramshaw},
  volume={6 6},
When provoked, Notaden bennetti frogs secrete an exudate which rapidly forms a tacky elastic solid ("frog glue"). This protein-based material acts as a promiscuous pressure-sensitive adhesive that functions even in wet conditions. We conducted macroscopic tests in air to assess the tensile strength of moist glue (up to 78 +/- 8 kPa) and the shear strength of dry glue (1.7 +/- 0.3 MPa). We also performed nanomechanical measurements in water to determine the adhesion (1.9-7.2 nN or greater… 

Biocompatibility and modification of the protein-based adhesive secreted by the Australian frog Notaden bennetti.

Dissolved glue successfully adhered collagen-coated perfluoropolyether lenses to debrided bovine corneas and supported epithelial regrowth and peroxidase treatment of dissolved glue introduced unnatural crosslinks between molecules of the major protein and resulted in the formation of a soft hydrogel, which was very different to the original material.

An Adhesive Secreted by Australian Frogs of the Genus Notaden

The properties of the frog glue suggest that a recombinant mimic would have great potential for medical applications, and in vitro, ex vivo and in vivo studies suggest that the structural matrix of the set glue is highly biocompatible.

Characterisation of a skin secretion with adhesive properties in the ground frog Eupsophus vertebralis (Alsodidae)

The sticky skin secretion released by the Patagonian frog Eupsophus vertebralis when stressed required a fast setting time to turn into strong adhesive, which worked well on synthetic and biological materials and suggest its biotechnological value for practical applications in industrial and medical sectors.

Proteinaceous adhesive secretions from insects, and in particular the egg attachment glue of Opodiphthera sp. moths.

Biochemical and electrophoretic screening of 29 adhesive secretions from Australian insects identified six types that appeared to consist largely of protein. Most were involved in terrestrial egg

Critical factors for the bulk adhesion of engineered elastomeric proteins

The strengths of proteins in this study were comparable to or higher than those of two commercially available protein-based adhesives, hide glue and a fibrin sealant, which may provide general rules for the design ofadhesives from elastomeric proteins.

Atomic force microscopy of the morphology and mechanical behaviour of barnacle cyprid footprint proteins at the nanoscale

Footprints deposited by Balanus amphitrite cyprids were probed by atomic force microscopy (AFM) in artificial sea water on silane-modified glass surfaces and revealed the fibrillar nature of the secretion, suggesting that the deposits were composed of single proteinaceous nanofibrils, or bundles of fibrils.

Mechanical properties of the cement of the stalked barnacle Dosima fascicularis (Cirripedia, Crustacea)

This study demonstrates that D. fascicularis cement is soft and visco-elastic, and shows that the values of the elastic modulus, hardness and tensile stress are considerably lower than in the rigid cement of other barnacles.

Biochemical analyses of the cement float of the goose barnacle Dosima fascicularis – a preliminary study

The goose barnacle Dosima fascicularis produces an excessive amount of adhesive (cement), which has a double function, being used for attachment to various substrata and also as a float (buoy). This



Post-Translational Modifications of Proteins

The purpose of this chapter is to attempt to compile a list of the amino acid derivatives known to exist in proteins in a manner that may be of use to protein chemists concerned with protein