Characterization of a porin channel in the endosymbiont of the trypanosomatid protozoan Crithidia deanei.

  title={Characterization of a porin channel in the endosymbiont of the trypanosomatid protozoan Crithidia deanei.},
  author={Iamara da Silva Andrade and Jo{\~a}o L{\'i}dio da Silva Gonçalves Vianez-J{\'u}nior and Carolina Lage Goulart and Fabrice Hombl{\'e} and Jean Marie Ruysschaert and Wanda Maria Almeida von Kr{\"u}ger and Paulo Mascarello Bisch and Wanderley de Souza and Ronaldo Mohana-Borges and Maria Cristina M. Motta},
  volume={157 Pt 10},
Crithidia deanei is a trypanosomatid protozoan that harbours a symbiotic bacterium. The partners maintain a mutualistic relationship, thus constituting an excellent model for studying metabolic exchanges between the host and the symbiont, the origin of organelles and cellular evolution. According to molecular analysis, symbionts of different trypanosomatid species share high identity and descend from a common ancestor, a β-proteobacterium of the genus Bordetella. The endosymbiont is surrounded… 
Expanded repertoire of kinetoplast associated proteins and unique mitochondrial DNA arrangement of symbiont-bearing trypanosomatids
Ulastructural analysis using high-resolution microscopy techniques revealed that the DNA fibrils are more compact in the kinetoplast region that faces the basal body and that the presence of the symbiotic bacterium does not interfere with kDNA topology.
The Symbiotic Bacterium Fuels the Energy Metabolism of the Host Trypanosomatid Strigomonas culicis.
Results of bioenergetic assays showed that the presence of the symbiont enhances the O2 consumption of the host cell, and inhibition of oxidative phosphorylation by potassium cyanide increased the rate of glycerol release and slightly diminished the ATP content in cells without the symbions, indicating that the host trypanosomatid enhances its fermentative activity when the bacterium is lost.
Structural Characterization of the Cell Division Cycle in Strigomonas culicis, an Endosymbiont-Bearing Trypanosomatid
Observations indicated that the association of the bacterium with the protozoan nucleus coordinates the cell cycle in both organisms.
Reduction of Tubulin Expression in Angomonas deanei by RNAi Modifies the Ultrastructure of the Trypanosomatid Protozoan and Impairs Division of Its Endosymbiotic Bacterium
Results indicate that the RNAi system is active in A. deanei and can be used to further explore gene function in symbiont‐containing trypanosomatids and to clarify important aspects of symbiosis and cell evolution.
Endosymbiosis in trypanosomatid protozoa: the bacterium division is controlled during the host cell cycle
The results indicate that host factors produced during the cell division cycle are essential for symbiont segregation and may control the bacterial cell number.
Importance of Angomonas deanei KAP4 for kDNA arrangement, cell division and maintenance of the host-bacterium relationship
Treatment with cisplatin and UV showed that Δkap4 null mutants were not more sensitive to DNA damage and repair than wild-type cells, suggesting that the kDNA in the kinetoplast of trypanosomatids has unique repair mechanisms.


Endosymbiosis in Trypanosomatids as a Model to Study Cell Evolution
Molecular data show that endosymbiont- harboring trypanosomatids represents an interesting model to study cell evolution, and according to rRNA sequences, symbiotic bacterium of different species are similar, being classified in the division of Proteobacteria, thus suggesting that a single evo- lutionary event gave rise to the symbiosis in the Trypanosomeidae family.
Ultrastructural and biochemical analysis of the relationship of Crithidia deanei with its endosymbiont.
Observations suggest that no electron transporting system is active in the symbionts of Crithidia deanei and that they might obtain energetic molecules derivated from the protozoan glycosomes.
Detection of Penicillin‐binding Proteins in the Endosymbiont of the Trypanosomatid Crithidia deanei
Results suggest that a physiologically active PBP is present in the cell envelope of C. deanei endosymbionts and may play important roles in the control of processes such as cell division and shape determination.
An endosymbiont positively modulates ornithine decarboxylase in host trypanosomatids.
Data indicate that the endosymbiont can enhance the protozoan ODC activity by providing factors of protein nature, which increase the host polyamine metabolism.
Effects of sterol biosynthesis inhibitors on endosymbiont-bearing trypanosomatids.
A sterol 24-methyltransferase inhibitor was used as a tool to investigate lipid biosynthetic pathways in Crithidia deanei, an endosymbiont-bearing trypanosomatid, and induced ultrastructural alterations, consisting of myelin-like figures in the cytoplasm and endosygiont envelope vesiculation.
Intracellular life.
A comparative analysis of Parasites and symbionts biology suggests some general considerations involved in adapting to intracellular life and reveals a number of independently achieved strategies for the exploitation of an intrACEllular habitat.
Protein synthesis in isolated symbionts from the Flagellate protozoon crithidia deanei
The inhibition of protein synthesis by the antibiotics provides direct evidence for the existence of a prokaryotic protein-synthesizing system in this unusual intracellular structure.
Trypanosomatidae: isoleucine requirement and threonine deaminase in species with and without endosymbionts.
Results indicate that, in trypanosomatids, only species harboring a symbiont are capable of isoleucine synthesis and that threonine deaminase, the first enzyme of the isoleUCine biosynthetic pathway, actually belongs to the symbionT.
Endosymbiont as supplier of ornithine carbamoyltransferase in a trypanosomatid
It is reported that endosymbionts enable certain species of Crithidia to synthesise arginine from ornithine, and this contribution to growth is significant because these species do not require haemm-synthesising enzymes for growth.
Solute channels of the outer membrane: from bacteria to chloroplasts
Comparison of structural and functional properties of chloroplast outer envelope and bacterial outer membrane channels is required to pinpoint the ancestral OEP ‘portrait gallery’.