Characterization of a novel thermostable (S)-amine-transaminase from an Antarctic moderately-thermophilic bacterium Albidovulum sp. SLM16.

  title={Characterization of a novel thermostable (S)-amine-transaminase from an Antarctic moderately-thermophilic bacterium Albidovulum sp. SLM16.},
  author={Sebasti{\'a}n L. M{\'a}rquez and J Atalah and Jenny M. Blamey},
  journal={Enzyme and microbial technology},
Discovery and Characterization of a Novel Thermostable β‐Amino Acid Transaminase from a Meiothermus Strain Isolated in an Icelandic Hot Spring
Ms‐TA2 is shown to be a thermostable enzyme by maintaining about 60% of the starting activity after 3 h incubation at 50 °C and showing a melting temperature of about 73 °C.
Novel transaminases from thermophiles: from discovery to application
Thermal characterization of stereoselective serine‐TAs confirmed they have excellent thermostability up to 60°C and high optimum temperatures and their Pfam predicted confirmed that TAs class I–II are the most abundant.
From the Discovery of Extremozymes to an Enzymatic Product: Roadmap Based on Their Applications
This brief research report describes three examples to illustrate a stepwise strategy for the development and production of commercial extremozymes, including a catalase from an Antarctic psychrotolerant microorganism, a laccase from a thermoalkaliphilic bacterium isolated from a hot spring and an amine-transaminases from a thermophilic bacterial site in Antarctica.


Identification of novel thermostable taurine–pyruvate transaminase from Geobacillus thermodenitrificans for chiral amine synthesis
A novel thermostable TPTA from Geobacillus thermodenitrificans is cloned and overexpressed in Escherichia coli and shows relatively good activity toward ketoses, suggesting its potential for catalyzing the asymmetric synthesis of chiral amino alcohols.
Purification and Characterization of a Novel (S)-Enantioselective Transaminase from Pseudomonas fluorescens KNK08-18 for the Synthesis of Optically Active Amines
Pseudomonas fluorescens KNK08-18, showing (S-selective transaminase activity, was isolated from soil by an enrichment culture method using (S)-7-methoxy-2-aminotetraline as the main nitrogen source and aminated a broad range of carbonyl compounds containing aromatic, non-aromatic, and acidic and non-acidic substrates.
Comparison of the ω-Transaminases from Different Microorganisms and Application to Production of Chiral Amines
Comparison of the enzyme properties indicates that ω-transaminase from V. fluvialis JS17 is the best one for both kinetic resolution and asymmetric synthesis to produce enantiomerically pure chiral amines.
Novel thermostable amine transferases from hot spring metagenomes
Functional characterization of the novel ATAs discovered in the metagenome of samples collected from hot springs in Iceland and in Italy demonstrated that they all possess a thermophilic character and are capable of performing amine transfer reactions using a broad range of donor and acceptor substrates, suggesting a good potential for practical synthetic applications.
Amine transaminases in chiral amines synthesis: recent advances and challenges
An update about the availability of microbial ATAs, discovered by both screening and database-mining approaches, or obtained by protein engineering of wild-type enzymes, will be provided, and innovative biocatalytic process strategies aimed at the improvement of ATAs performances in chiral amines synthesis will be presented.
A novel transaminase, (R)-amine:pyruvate aminotransferase, from Arthrobacter sp. KNK168 (FERM BP-5228): purification, characterization, and gene cloning
The deduced amino acid sequence was found to be homologous to those of the aminotransferases belonging to fold class IV of pyridoxal-5′-phosphate-dependent enzymes, such as branched-chain amino acid aminotsferases.
Isolation and partial characterization of a new moderate thermophilic Albidovulum sp. SLM16 with transaminase activity from Deception Island, Antarctica
Strain SLM16 is a moderate thermophilic Gram negative microorganisms which belongs to the marine bacterial genus Al bidovulum and is closely related to Albidovulum inexpectatum species based on phylogenetic analysis.
Microbial synthesis of chiral amines by (R)-specific transamination with Arthrobacter sp. KNK168
(R)-1-Phenylethylamine was a good amino donor for amination of 3,4-dimethoxyphenylacetone with Arthrobacter sp.
Bacillus anthracis ω-amino acid:pyruvate transaminase employs a different mechanism for dual substrate recognition than other amine transaminases
Function insights for the B. anthracis enzyme are provided, function predictions of related proteins are enabled, and knowledge regarding ω-amino acid and amine converting transaminases is broadened.