Characterization of a novel salt-, xylose- and alkali-tolerant GH43 bifunctional β-xylosidase/α-l-arabinofuranosidase from the gut bacterial genome.

  title={Characterization of a novel salt-, xylose- and alkali-tolerant GH43 bifunctional $\beta$-xylosidase/$\alpha$-l-arabinofuranosidase from the gut bacterial genome.},
  author={Bo Xu and Liming Dai and Wenhong Zhang and Yunjuan Yang and Qian Wu and Junjun Li and Xianghua Tang and Junpei Zhou and Junmei Ding and Nanyu Han and Zunxi Huang},
  journal={Journal of bioscience and bioengineering},
A GH43 bifunctional β-xylosidase encoding gene (XylRBM26) was cloned from Massilia sp. RBM26 and successfully expressed in Escherichia coli. Recombinant XylRBM26 exhibited β-xylosidase and α-l-arabinofuranosidase activities. When 4-nitrophenyl-β-d-xylopyranoside was used as a substrate, the enzyme reached optimal activity at pH 6.5 and 50°C and remained stable at pH 5.0-10.0. Purified XylRBM26 presented good salt tolerance and retained 96.6% activity in 3.5 M NaCl and 77.9% initial activity… 
Molecular and Biochemical Characterization of Salt-Tolerant Trehalose-6-Phosphate Hydrolases Identified by Screening and Sequencing Salt-Tolerant Clones From the Metagenomic Library of the Gastrointestinal Tract
This study is the first to report the identification as well as molecular and biochemical characterization of salt-tolerant trehalose-6-phosphate hydrolase from the metagenomic library of the gastrointestinal tract.
β-Xylosidases: Structural Diversity, Catalytic Mechanism, and Inhibition by Monosaccharides
The structural diversity and catalytic mechanisms of β-xylosidases are reviewed, and their inhibition by monosaccharides is discussed.
Two distinct catalytic pathways for GH43 xylanolytic enzymes unveiled by X-ray and QM/MM simulations
Functional, structural and computational characterization of a GH43 enzyme, including a snapshot of an active Michaelis complex, reveal the hydrolysis mechanism and suggest two possible reaction pathways, involving different transition state conformations for the cleavage of xylooligosaccharides.
Arabinofuranosidases: Characteristics, microbial production, and potential in waste valorization and industrial applications.
The main aim of this review is to highlight the important properties of this interesting enzyme, microorganisms used for its production, and enhanced production using genetic engineering approach and an outlook on further research and development.
Recent advances on key enzymatic activities for the utilisation of lignocellulosic biomass.
A summary of the progress in the field is presented, including recent discoveries on the nature of the structural polysaccharides, new technologies for the discovery and functional annotation of gene sequences, and the novel lignocellulose-acting enzymes described.
Mining saltmarsh sediment microbes for enzymes to degrade recalcitrant biomass
The recalcitrance of biomass represents a major bottleneck for the efficient production of fermentable sugars from biomass. Cellulase cocktails are often only able to release 75-80% of the potential
Molecular modification, structural characterization, and biological activity of xylans.
The major molecular modification methods of xylans are introduced, and the primary structure and conformation characteristics ofxylans and their derivatives are summarized and the biological activity and structure-activity relationship of the modified xylan are discussed.


Purification and biochemical properties of a thermostable xylose-tolerant β-D-xylosidase from Scytalidium thermophilum
The thermophilic fungus Scytalidium thermophilum produced large amounts of periplasmic β-D-xylosidase activity when grown on xylan as carbon source, and this enzyme was purified using a procedure that included heating at 50°C, ammonium sulfate fractioning, and chromatography on Sephadex G-100 and DEAE-SephadeX A-50.
Characterization of a recombinant xylose tolerant β-xylosidase from Humicola grisea var. thermoidea and its use in sugarcane bagasse hydrolysis.
HXYLA is distinguished from other β-xylosidases by the attractive characteristics for industrial applications such as thermostability, high tolerance xylose and saccharification of biomass by convert xylan into fementable monosaccharides and improve cellulose hydrolysis.
Biochemical properties of a novel thermostable and highly xylose-tolerant β-xylosidase/α-arabinosidase from Thermotoga thermarum
A useful and novel β-xylosidase displaying extraordinary and desirable properties: high xylose tolerance and catalytic activity at temperatures above 75°C, thermally stable and excellent hydrolytic activity on xylooligosaccharides.
A halotolerant bifunctional β-xylosidase/α-l-arabinofuranosidase from Colletotrichum graminicola: Purification and biochemical characterization.
This is the first report of a halotolerant bifunctional β-xylosidase/α-l-arabinofuranosidase from C. graminicola, and the enzyme showed attractive properties for application in lignocellulose hydrolysis, particularly under high salinity and/or in the presence of residues of pretreatment steps.
Production and Characteristics of a Novel Xylose- and Alkali-tolerant GH 43 β-xylosidase from Penicillium oxalicum for Promoting Hemicellulose Degradation
The novel intracellular glycoside hydrolase family 43 β-xylosidase gene (xyl43) from Penicillium oxalicum 114-2 was successfully high-level overexpressed in Pichia pastoris, and the secreted enzyme was characterized and exhibited great pH stability and high catalytic activity.
High Level Expression of a Novel Family 3 Neutral β-Xylosidase from Humicola insolens Y1 with High Tolerance to D-Xylose
A novel β-xylosidase gene of glycosyl hydrolase (GH) family 3, xyl3A, was identified from the thermophilic fungus Humicola insolens Y1, which is an innocuous and non-toxic fungus that produces a wide
Two xylose-tolerant GH43 bifunctional β-xylosidase/α-arabinosidases and one GH11 xylanase from Humicola insolens and their synergy in the degradation of xylan.
This study provides several enzymes for synergistic degradation of xylan and contributes to the formulation of optimised enzyme mixtures for the efficient hydrolysis of plant biomass.
Biochemical and kinetic characterisation of a novel xylooligosaccharide-upregulated GH43 β-d-xylosidase/α-l-arabinofuranosidase (BXA43) from the probiotic Bifidobacterium animalis subsp. lactis BB-12
Multiple sequence alignment and homology modelling indicated that Arg505Tyr506 present in BXA43 are probably important for binding to xylotetraose at subsite +3 and occur only in GH43 from the Bifidobacterium genus.
Applicability of recombinant β-xylosidase from the extremely thermophilic bacterium Geobacillus thermodenitrificans in synthesizing alkylxylosides.
The β-xylosidase encoding gene (XsidB) of the extremely thermophilic bacterium Geobacillus thermodenitrificans has been cloned and expressed in Escherichia coli, and the structure was confirmed by (1)H NMR analysis.
Screening, identification, and characterization of a GH43 family β-xylosidase/α-arabinofuranosidase from a compost microbial metagenome
Results indicate that CoXyl43 exhibits unique enzymatic properties useful for biomass saccharification.