Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae.

Abstract

The promoter of the Streptococcus pneumoniae putative fuculose kinase gene (fcsK), the first gene of a novel fucose utilization operon, is induced by fucose and repressed by glucose or sucrose. When the streptococcal polypeptide deformylase (PDF) gene (def1, encoding PDF) was placed under the control of P(fcsK), fucose-dependent growth of the S. pneumoniae (P(fcsK)::def1) strain was observed, confirming the essential nature of PDF in this organism. The mode of antibacterial action of actinonin, a known PDF inhibitor, was also confirmed with this strain. The endogenous fuculose kinase promoter is a tightly regulated, titratable promoter which will be useful for target validation and for confirmation of the mode of action of novel antibacterial drugs in S. pneumoniae.

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@article{Chan2003CharacterizationOA, title={Characterization of a novel fucose-regulated promoter (PfcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae.}, author={Pan Chan and Karen M O'Dwyer and Leslie M Palmer and Jennifer D Ambrad and Karen A Ingraham and Chi Y So and Michael A Lonetto and Sanjoy Biswas and Martin E. Rosenberg and David J. Holmes and Magdalena Zalaca{\'i}n}, journal={Journal of bacteriology}, year={2003}, volume={185 6}, pages={2051-8} }