Characterization of a novel cytochrome P450 from Amycolatopsis sp. CGMCC1149 for hydroxylation of lovastatin

@article{Zong2015CharacterizationOA,
  title={Characterization of a novel cytochrome P450 from Amycolatopsis sp. CGMCC1149 for hydroxylation of lovastatin},
  author={H. Zong and B. Zhuge and Xinyao Lu and X. Huo and H. Fang and Jian Song and J. Sun},
  journal={Biotechnology and Applied Biochemistry},
  year={2015},
  volume={62}
}
  • H. Zong, B. Zhuge, +4 authors J. Sun
  • Published 2015
  • Biology, Medicine
  • Biotechnology and Applied Biochemistry
Wuxistatin, a novel and potent statin, is converted from lovastatin by Amycolatopsis sp. CGMCC1149. In the bioconversion, lovastatin is firstly hydroxylated to 3‐hydroxymethyl lovastatin (product I) by a hydroxylase. In the current study, a novel hydroxylase gene p450lov was isolated from Amycolatopsis sp. CGMCC1149 by degenerate PCR and self‐formed adaptor PCR and expressed in Escherichia coli. The gene encodes a 403‐amino‐acid protein with a molecular weight of 44.8 kDa and was designated as… Expand
Secondary Metabolites of the Genus Amycolatopsis: Structures, Bioactivities and Biosynthesis
TLDR
The objective in this review is to summarize the chemical structures and biological activities of secondary metabolites from the genus Amycolatopsis, a rare actinomycete genus with the potential to produce antibiotics with a total of 159 compounds derived from 8 known and 18 unidentified species. Expand
Detection of 4a,5-dihydropravastatin as Impurity in the Cholesterol Lowering Drug Pravastatin
TLDR
In fermentation broth samples of the single step production of pravastatin, 4a,5-dihydro analogues are detected and identified for the first time and it is confirmed that after several recrystallization steps this impurity can be fully removed from the pravastsatin powder. Expand

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