Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus

  title={Characterization of a novel chitinase, DkChi, from Dendrolimus kikuchii nucleopolyhedrovirus},
  author={Qinghua Wang and Liangjian Qu and Zhilin Zhang and Yuzhu Wang and Yongan Zhang},
  journal={Archives of Virology},
Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii. In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the genomic DNA of DkNPV using a DNA fragment library. The DkChi gene, encoding 558 residues protein with a predicted mass of 61.6 kDa, was expressed at high levels in Escherichia coli and purified by affinity… CONTINUE READING


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