Characterization of a novel Negevirus and a novel Bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad.

@article{Auguste2014CharacterizationOA,
  title={Characterization of a novel Negevirus and a novel Bunyavirus isolated from Culex (Culex) declarator mosquitoes in Trinidad.},
  author={Albert J. Auguste and Christine V. F. Carrington and Naomi L Forrester and Vsevolod L. Popov and Hilda Guzm{\'a}n and Steven G. Widen and Thomas G. Wood and Scott C. Weaver and Robert B. Tesh},
  journal={The Journal of general virology},
  year={2014},
  volume={95 Pt 2},
  pages={
          481-5
        }
}
Pools of mosquitoes were tested for insect-specific viruses using cytopathic effect (CPE) assays on Aedes albopictus (C6/36) cells. Illumina sequencing of RNA from pool TR7094, which produced extensive CPE 2 days post-infection, yielded the complete genome sequences of a previously unknown Bunyavirus, designated Cumuto virus (CUMV), and a second virus designated Wallerfield virus (WALV). WALV shared highest amino acid identity (60.1 %) with Dezidougou virus from Côte d'Ivoire, a positive-sense… Expand
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