Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector

@article{Tsuchida2008CharacterizationOA,
  title={Characterization of a new 2.4-kb plasmid of Corynebacterium casei and development of stable corynebacterial cloning vector},
  author={Yoshiki Tsuchida and Sakurako Kimura and N Suzuki and Masayuki Inui and Hideaki Yukawa},
  journal={Applied Microbiology and Biotechnology},
  year={2008},
  volume={81},
  pages={1107-1115}
}
A new plasmid pCASE1 was isolated from Gram-positive Corynebacterium casei JCM 12072. It comprised a 2.4-kb nucleotide sequence with three ORFs, two of which were indispensable for autonomous replication in Corynebacterium glutamicum. Homology search identified these two ORFs as repA and repB, areas coding proteins involved in plasmid replication. repA sequence showed high similarity to theta-replicating Escherichia coli ColE2-P9 plasmids and even higher similarity to plasmids derived from Gram… CONTINUE READING