Characterization of a mutant of Lactococcus lactis with reduced membrane-bound ATPase activity under acidic conditions.

@article{Amachi1998CharacterizationOA,
  title={Characterization of a mutant of Lactococcus lactis with reduced membrane-bound ATPase activity under acidic conditions.},
  author={Seigo Amachi and Kazuhiko Ishikawa and Shuichi Toyoda and Yasuo Kagawa and Atsushi Yokota and Fusao Tomita},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1998},
  volume={62 8},
  pages={1574-80}
}
A mutant of Lactococcus lactis subsp. lactis C2 with reduced membrane-bound ATPase activity was characterized to clarify its acid sensitivity. The cytoplasmic pH of the mutant was measured in reference to the parental strain under various pH conditions. At low pH, the mutant could not maintain its cytoplasmic pH near neutral, and lost its viability faster than the parental strain. The ATPase activities of cells cultured under neutral and acidic conditions using pH-controlled jar fermentors were… CONTINUE READING

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