Characterization of a monoclonal antibody that neutralizes the activity of prostaglandin E2.

@article{Mnich1995CharacterizationOA,
  title={Characterization of a monoclonal antibody that neutralizes the activity of prostaglandin E2.},
  author={Stephen J. Mnich and A W Veenhuizen and Joseph B. Monahan and Kathleen C. F. Sheehan and Kevin R Lynch and Peter C. Isakson and Joseph P. Portanova},
  journal={Journal of immunology},
  year={1995},
  volume={155 9},
  pages={4437-44}
}
We describe the generation of an IgG1 mAb, 2B5, that neutralizes the biologic activity of PGE2 in vitro and in vivo. The Ab was derived from a BALB/c mouse that was immunized with a PGE2-thyroglobulin conjugate. 2B5 is one of the highest affinity and specific anti-PGE2 mAbs reported to date. The Kd for PGE2 was approximately 300 pM and crossreactivity toward eicosanoids other than PGE1 was less than 1%. The addition of 2B5 to [3H]PGE2 blocked the binding of radioligand to cell membranes… CONTINUE READING

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