Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells

@article{Kuriyama1995CharacterizationOA,
  title={Characterization of a minus end-directed kinesin-like motor protein from cultured mammalian cells},
  author={Ryoko Kuriyama and Matthew Kofron and Russell Essner and Toshiro Kato and S Dragas-Granoic and Charlotte K. Omoto and Alexey Khodjakov},
  journal={The Journal of Cell Biology},
  year={1995},
  volume={129},
  pages={1049 - 1059}
}
Using the CHO2 monoclonal antibody raised against CHO spindles (Sellitto, C., M. Kimble, and R. Kuriyama. 1992. Cell Motil. Cytoskeleton. 22:7-24) we identified a 66-kD protein located at the interphase centrosome and mitotic spindle. Isolated cDNAs for the antigen encode a 622-amino acid polypeptide. Sequence analysis revealed the presence of 340-amino acid residues in the COOH terminus, which is homologous to the motor domain conserved among other members of the kinesin superfamily. The… CONTINUE READING

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