Characterization of a methyl jasmonate and wounding-responsive cytochrome P450 of Arabidopsis thaliana catalyzing dicarboxylic fatty acid formation in vitro.

@article{Kandel2007CharacterizationOA,
  title={Characterization of a methyl jasmonate and wounding-responsive cytochrome P450 of Arabidopsis thaliana catalyzing dicarboxylic fatty acid formation in vitro.},
  author={Sylvie Kandel and Vincent Sauveplane and Vincent Compagnon and Rochus Benni Franke and Yves A. Millet and Lukas Schreiber and Daniele Werck-reichhart and Franck Pinot},
  journal={The FEBS journal},
  year={2007},
  volume={274 19},
  pages={5116-27}
}
A fatty-acid-metabolizing enzyme from Arabidopsis thaliana, CYP94C1, belonging to the cytochrome P450 family was cloned and characterized. CYP94C1 was heterologously expressed in a Saccharomyces cerevisiae strain (WAT11) engineered for P450 expression. When recombinant yeast microsomes were incubated with lauric acid (C12:0) for 15 min, one major metabolite was formed. The product was purified and identified by GC/MS as 12-hydroxylauric acid. Longer incubation (40 min) led to the formation of… CONTINUE READING

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