Characterization of a mast cell line that lacks the extracellular domain of membrane c-kit.

@article{Mekori1997CharacterizationOA,
  title={Characterization of a mast cell line that lacks the extracellular domain of membrane c-kit.},
  author={Yoseph A. Mekori and Chad K. Oh and Jaroslaw Dastych and Julie P. Goff and Souichi Adachi and Peter J. Bianchine and Alexandra S. Worobec and Tekli Semere and Jacalyn H. Pierce and Dean D Metcalfe},
  journal={Immunology},
  year={1997},
  volume={90 4},
  pages={518-25}
}
Expression of the c-kit proto-oncogene receptor on mast cells is essential for their normal proliferation and maturation as well as for several biological responses such as chemotaxis and attachment. In the present study we report that the interleukin-3 (IL-3)-dependent mast cell line CFTL-15 lacks the extracellular domain of the c-kit receptor. This observation was made after noting that the c-kit ligand stem cell factor (SCF) could not prevent IL-3 deprivation-induced mast cell apoptosis and… CONTINUE READING