Characterization of a major surface-associated excretory-secretory antigen of Trichinella spiralis larvae with antibodies to keyhole limpet haemocyanin.

Abstract

A multi-subunit antigen (native M(r) > 200 kDa, reduced M(r) 97-100 kDa) has been identified in homogenates of Trichinella spiralis larvae using affinity-purified rabbit anti-keyhole limpet haemocyanin (KLH) antibodies and its cross-reactivity with KLH was confirmed by competition blotting. The antigen was not present at the larval surface but was exposed… (More)

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