Characterization of a major permeability barrier in the zebrafish embryo.

  title={Characterization of a major permeability barrier in the zebrafish embryo.},
  author={Mary Hagedorn and Frederick W. Kleinhans and Dmitri Artemov and Ulrich Pilatus},
  journal={Biology of reproduction},
  volume={59 5},
Fish embryos represent a class of multicompartmental biological systems that have not been successfully cryopreserved, primarily because of the lack of understanding of how water and cryoprotectants permeate the compartments. We are using the zebrafish embryo as a model to understand these kinetics. Zebrafish embryos have two major compartments, the blastoderm and the yolk, which is surrounded by the multinucleated yolk syncytial layer (YSL). We determined the water and cryoprotectant… CONTINUE READING