Characterization of a low-level unknown isomeric degradation product using an integrated online-offline top-down tandem mass spectrometry platform.

@article{Yu2013CharacterizationOA,
  title={Characterization of a low-level unknown isomeric degradation product using an integrated online-offline top-down tandem mass spectrometry platform.},
  author={Xiang Yu and Christopher Warme and Dinah Lee and Jing Zhang and Wendy Zhong},
  journal={Analytical chemistry},
  year={2013},
  volume={85 19},
  pages={8964-7}
}
An integrated online-offline platform was developed combining automated online LC-MS fraction collection, continuous accumulation of selected ions (CASI), and offline top-down electron capture dissociation (ECD) tandem mass spectrometry experiments to identify a low-level, unknown isomeric degradant in a formulated drug product during an accelerated stability study. By identifying the diagnostic ions of the isoaspartic acid (isoAsp), the top-down ECD experiment showed that the Asp9 in exenatide… CONTINUE READING