Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis

  title={Characterization of a human regulatory subunit of protein phosphatase 3 gene (PPP3RL) expressed specifically in testis},
  author={Lingling Liu and Jianxuan Zhang and Jian-Li Yuan and Yongjun Dang and Chenyi Yang and Xiujuan Chen and Jianing Xu and Long Yu},
  journal={Molecular Biology Reports},
AbstractProtein phosphatase 3 (PPP3, formerly PP2B, Calcineurin), a serine/ threonine protein phosphatase, is a heterodimer composed of one catalytic subunit (PPP3C, Calcineurin A) and one regulatory subunit (PPP3R, Calcineurin B). PPP3R, an EF-hand Ca2+ binding protein, contains four high-affinity EF-hand calcium-binding sites, indicating that PPP3 plays critical roles in many calcium-mediated signal transduction pathways. PPP3R has two isoforms, PPP3R1 (also known as PP2Bβ1) and PPP3R2 (also… 
Partial Inhibition of Calcineurin Activity by Rcn2 as a Potential Remedy for Vps13 Deficiency
It is shown that overexpression of the RCN2 gene, which encodes a negative regulator of calcineurin, is beneficial for vps13Δ cells and the molecular mechanism underlying this effect through site-directed mutagenesis of RCN1 and Rcn2 is studied.
Calcineurin tax-6 regulates male ray development and counteracts with kin-29 kinase in Caenorhabditis elegans
It is suggested that balanced phosphorylation mediated by tax-6 and kin-29 is required for proper development of T lineage rays, and tax- 6 and sma-6 may function in a parallel pathway in the developmental process of V5 lineage ray 1.
Direct Evidence for Calcineurin Binding to the Exon-7 Loop of the Sodium-Bicarbonate Cotransporter NBCn1
A new perspective on concurrent studies of NBCn1 in various tissues is provided by using cell-free binding assays to investigate the interaction ofNBCn1 with the calcineurin (Cn) isoform PPP3CA, and the protein domain Exon 7 binds Cn with an equilibrium dissociation constant of 30 +/- 15 nm.
Molecular analysis of CIB4 gene and protein in Kermani sheep
Although it was demonstrated that CIB4 is a testis-specific gene, expressed only in the testis and it interacts with other proteins, the mechanisms by which C IB4 expression is regulated need to be elucidated.
Calcineurin inactivation inhibits pyruvate dehydrogenase complex activity and induces the Warburg effect
It is shown that calcineurin interacts with pyruvate dehydrogenase complex (PDC), a mitochondrial gatekeeper enzyme that connects two key metabolic pathways of cells, glycolysis and the tricarboxylic acid cycle, and suggests that CNIs may promote tumorigenesis through inhibition of the calcineURin-PDC pathway.
Calcineurin is required for male sex pheromone biosynthesis and female acceptance
It is demonstrated that HaCNA acts as downstream signal of PBAN/Ca2+ and plays an important role in PBAN‐induced male sex pheromone biosynthesis and female mating acceptance.
The effect of calcineurin inhibition on the expression and activation of renal electrolyte transporters
A novel mechanism explaining CNI-induced hypercalciuria, driven by the activation of NCC, is proposed and the relationship between sodium and calcium handling in the DCT is discussed.
The Immunosuppressant Brasilicardin: Determination of the Biosynthetic Gene Cluster in the Heterologous Host Amycolatopsis japonicum.
Investigation of the genes flanking the previously defined brasilicardin biosynthetic gene cluster revealed two novel genes (bra0, bra12), which are involved in brasilicARDin biosynthesis: bra12 encodes a transcriptional activator of the brasicardin gene cluster and bra0 codes for a dioxygenase involved in methoxylation of brasilicards.


Calcineurin A alpha (PPP3CA), calcineurin A beta (PPP3CB) and calcineurin B (PPP3R1) are located on human chromosomes 4, 10q21-->q22 and 2p16-->p15 respectively.
This enzyme can play a critical role in transcriptional regulation and growth control in T lymphocytes by a mechanism believed to involve dephosphorylation of the nuclear factor NF-AT which is essential for transcription of the interleukin-2 gene.
Regulation of the Calmodulin-stimulated Protein Phosphatase, Calcineurin*
The demonstration that FK506 and CsA, when bound to their respective binding proteins, are specific inhibitors of calcineurin provided the tools needed to reveal its many other roles in the transduction of Ca signals.
Calcineurin: not just a simple protein phosphatase.
  • D. Guerini
  • Biology, Chemistry
    Biochemical and biophysical research communications
  • 1997
The protein sequence and crystallographic structure suggest that the catalytic subunit of calcineurin has high homologies with other protein phosphatases, and the understanding of the role of calcinesurin in other cells, in particular neurons, is also progressing rapidly.
Isolation and sequence of a cDNA clone for human calcineurin B, the Ca2+-binding subunit of the Ca2+/calmodulin-stimulated protein phosphatase.
As was observed with protein levels, mRNA abundance in brain is 20-60 times that found in other tissues with the exception of HeLa cells which, like brain, contain abundant calcineurin B mRNA.
Distribution and activity of calcineurin in rat tissues. Evidence for post-transcriptional regulation of testis-specific calcineurin B.
Investigation of the distribution and activity ofCalcineurin in rat tissues demonstrates that CN is a widely distributed protein phosphatase and that its activity is regulated in a tissue-specific manner.