Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties.

@article{Rodan1987CharacterizationOA,
  title={Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties.},
  author={Sevgi B. Rodan and Yoichi Imai and Mark A. Thiede and G. Wesolowski and Dawn Thompson and Zvi Bar-Shavit and Sara J Shull and Klaus Mann and Gideon A. Rodan},
  journal={Cancer research},
  year={1987},
  volume={47 18},
  pages={4961-6}
}
This study examines the osteoblastic properties of the established human osteosarcoma cell line Saos-2. Saos-2 cells inoculated into diffusion chambers, which were implanted i.p. into nude mice, produced mineralized matrix in 4 of 6 chambers at 8 weeks. In 5 of 6 chambers there was a strong positive alkaline phosphatase reaction. In culture the alkaline phosphatase levels increased with time and cell density, reaching very high levels at confluence: 4-7 mumol/mg protein/min. The cells show a… CONTINUE READING
123 Citations
40 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 123 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 40 references

l o , 25 - Dihydroxyvitamin I ) , specific regulation of growth , morphology and fibronectin in a human osteosarcoma cell line

  • R. T.. James Franchesi, M. W., G. Zerlauth
  • J . Cell . Physiol .
  • 1985

Biosynthesis of osteonectin by fetal porcine calvarÃ-a!cells in vitro

  • K. Otsuka, Yao, +6 authors J. D. Termine
  • J. Biol. Chem.,
  • 1984
3 Excerpts

Expression of the osteoblastic phenotype

  • A Rodan.G., S. B. Rodan
  • Advances in Bone and Mineral Research,
  • 1984
3 Excerpts

Similar Papers

Loading similar papers…