Characterization of a human monoclonal autoantibody directed to cardiolipin/β2 glycoprotein I produced by chronic lymphocytic leukaemia B cells

@article{Mariette1993CharacterizationOA,
  title={Characterization of a human monoclonal autoantibody directed to cardiolipin/$\beta$2 glycoprotein I produced by chronic lymphocytic leukaemia B cells},
  author={Xavier Mariette and Yves L{\'e}vy and M L Dubreuil and Liliane Intrator and Françoise Danon and Jean Claude Brouet},
  journal={Clinical \& Experimental Immunology},
  year={1993},
  volume={94}
}
We determined the specificity and sequence of immunoglobulin molecules synthesized by monoclonal B cells from a patient with chronic lymphocytic leukaemia (CLL) who presented with a number of clinical and biological autoimmune symptoms. Helerohybrids obtained by fusion of CLL cells with the mouse X63‐Ag 8.653 myeloma produced IgMλ MoAbs directed to the cardiolipin/β2 glycoprotein I (β2GPI) complex and ssDNA. They were devoid of polyreactivity. Nuclcotide sequence analysis of the variable domain… 
Immunoglobulin gene sequence analysis of anti-cardiolipin and anti-cardiolipin idiotype (H3) human monoclonal antibodies.
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Gene sequences which encode the disease-associated H3 idiotype and its location on lambda light chains, which imply that some labda light chains may be preferentially utilized in auto-reactive hybridomas, are shown.
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TLDR
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TLDR
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Molecular properties of human monoclonal anti-DNA and antiphospholipid antibodies.
TLDR
Analysis of the sequences of monoclonal anti-dsDNA and APL derived from humans or mice suggests that these binding properties are derived by antigen driven accumulation of mutations in the complementarity determining regions (CDRs) of the heavy and Ight chains.
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