Characterization of a hepatitis B and hepatitis delta virus receptor binding site

  title={Characterization of a hepatitis B and hepatitis delta virus receptor binding site},
  author={M Engelke and Kerry Mills and Stefan Seitz and Petra Simon and Philippe Gripon and Martina Schnölzer and Stephan Urban},
Insights into the early infection events of the human hepatitis B (HBV) and hepatitis delta virus (HDV) have been limited because of the lack of a cell culture system supporting the full replication cycle for these important pathogens. The human hepatoma cell line HepaRG allows the experimental induction of a differentiated state, thereby gaining susceptibility toward HBV and HDV infection. We recently identified HBV envelope protein–derived lipopeptides comprising amino acids 2 though 48 of… 
Entry of hepatitis B and hepatitis D virus into hepatocytes: Basic insights and clinical implications
Entry of hepatitis B virus: mechanism and new therapeutic target.
Two Potentially Important Elements of the Hepatitis B Virus Large Envelope Protein Are Dispensable for the Infectivity of Hepatitis Delta Virus
ABSTRACT Previous studies have attempted to clarify the roles of the pre-S1 and pre-S2 domains of the large envelope protein of hepatitis B virus (HBV) in attachment and entry into susceptible cells.
Efficient Inhibition of Hepatitis B Virus Infection by a preS1-binding Peptide
The 4B10-mediated inhibition of HBV infection is specific as it did not inhibit the infection of vesicular stomatitis virus glycoprotein pseudotyped lentivirus or human immunodeficiency virus type 1.
Fine Mapping of Pre-S Sequence Requirements for Hepatitis B Virus Large Envelope Protein-Mediated Receptor Interaction
The data suggest that HBV pre-S1-mediated receptor interference and, thus, HBV receptor recognition form a highly specific process that requires an N-terminal acyl moiety and a highly conserved sequence that is present in primate but not rodent or avian hepadnaviruses.
New insights into hepatitis B and hepatitis delta virus entry
It is known that the large viral surface protein (L) plays a pivotal role in HBV entry, and mediates diverse functions, commencing binding of virions to heparan sulfate proteoglycans at the hepatocytes surface as a prerequisite for entry.
Strategies to inhibit entry of HBV and HDV into hepatocytes.
It is important to study the therapeutic potential of virus entry inhibitors, especially when combined with strategies to induce immune-mediated killing of infected hepatocytes.
Assembly of Hepatitis B Virus Envelope Proteins onto a Lentivirus Pseudotype That Infects Primary Human Hepatocytes
It is demonstrated that the envelope proteins of hepatitis B virus (HBV) could be incorporated into the lipid membrane of lentivirus pseudotype particles and the infection of PHH by the pseudotype was sensitive to known inhibitors of HBV and HDV entry.
Assembly of Hepatitis Delta Virus: Particle Characterization, Including the Ability To Infect Primary Human Hepatocytes
This HDV assembly and infection system can be further developed to better understand the mechanisms shared by HBV and HDV for attachment and entry into host cells.
Hepatitis B Virus and Hepatitis D Virus Entry, Species Specificity, and Tissue Tropism.
This review summarizes the current understanding of the mechanisms of HBV/HDV entry and indicates that sodium taurocholate cotransporting polypeptide (NTCP) is at least one of the factors determining the narrow species specificity and hepatotropism ofHBV and HDV.


Mapping of the Hepatitis B Virus Pre-S1 Domain Involved in Receptor Recognition
The ability of short pre-S1 peptides to efficiently inhibit HDV infection suggests that they represent suitable ligands for identification of the HBV receptor and that a pre- S1 mimetic may represent a rational therapy for the treatment of HBV infection.
Role of the Antigenic Loop of the Hepatitis B Virus Envelope Proteins in Infectivity of Hepatitis Delta Virus
Results demonstrate that in addition to a receptor-binding site previously identified in the pre-S1 domain of the L protein, a determinant of infectivity resides in the antigenic loop of HBV envelope proteins.
Myristylation of the hepatitis B virus large surface protein is essential for viral infectivity.
It is found that mutant viral particles contained viral DNA with a reduced mean size, probably corresponding to a larger single-stranded region in the relaxed circular DNA form, and that mutant viruses were normally assembled.
Avian Hepatitis B Virus Infection Is Initiated by the Interaction of a Distinct Pre-S Subdomain with the Cellular Receptor gp180
It is shown that the functionality of the DHBV pre-S subdomain, which interacts with the cellular receptor, is determined predominantly by a defined three-dimensional structure rather than by primary sequence elements, and that cellular uptake of hepadnaviruses is a multistep process involving more than a single cellular receptor component.
Inhibition of Duck Hepatitis B Virus Infection by a Myristoylated Pre-S Peptide of the Large Viral Surface Protein
The duck hepatitis B virus model is used to study the interference with infection by a myristoylated peptide representing an N-terminal pre-S subdomain of the large viral envelope protein, which is related to the postulated host-discriminating cofactor of infection.
Mapping of the hepatitis B virus attachment site by use of infection-inhibiting preS1 lipopeptides and tupaia hepatocytes.
Findings indicate that the current subunit hepatitis B vaccines may be improved by the addition of distinct preS1 epitopes, and preS 1 lipopeptides are promising candidates for specific antiviral therapy against hepatitis B infections.
Role of the Pre-S2 Domain of the Large Envelope Protein in Hepatitis B Virus Assembly and Infectivity
It is indicated that only 10% of the large-protein pre-S2 region at its N-terminal extremity is essential for virion export and that the remaining part, dispensable for viral secretion, is also dispensability for infectivity.
Pre-S1 Antigen-Dependent Infection of Tupaia Hepatocyte Cultures with Human Hepatitis B Virus
It is shown that purified HBV infects primary T. belangeri hepatocyte cultures in a very specific manner, as detected by HBV covalently closed circular DNA, mRNA, HBV e antigen, and HBsAg production.
Production of hepatitis B virus particles in Hep G2 cells transfected with cloned hepatitis B virus DNA.
The Hep G2 cell line can support the assembly and secretion not only of several of the replicative intermediates of HBV DNA but also of Dane-like particles, and this in vitro system can now be used to study the life cycle ofHBV and the reaction of immunocompetent cells with cells carrying HBV.
Large surface proteins of hepatitis B virus containing the pre-s sequence
Immunological cross-reactions showed that P39/GP42 is partially homologous to P24/GP27 and GP33/GP36, and a model is presented of how the open reading frame for the viral envelope leads to defined amounts of three different proteins.