Characterization of a heparin-binding growth factor from adenocarcinoma of the kidney.

@article{Mydlo1988CharacterizationOA,
  title={Characterization of a heparin-binding growth factor from adenocarcinoma of the kidney.},
  author={Jack H. Mydlo and Warren DeWayne Heston and William R. Fair},
  journal={The Journal of urology},
  year={1988},
  volume={140 6},
  pages={1575-9}
}
A polypeptide isolated from tissue extracts of renal adenocarcinoma was mitogenic for BALB/c 3T3 cells and human umbilical vein (HUV) cells in culture. It also demonstrated angiogenic ability using the chorioallantoic membrane bioassay. Using heparin-sepharose affinity chromatography the purified protein eluted with a NaCl concentration between 1.4 and 1.8 M and demonstrated a molecular weight of approximately 17,000 daltons based on SDS polyacrylamide gel electrophoresis. Half maximal… CONTINUE READING