Characterization of a gene cluster for exopolysaccharide biosynthesis and virulence in Erwinia stewartii.

@article{Dolph1988CharacterizationOA,
  title={Characterization of a gene cluster for exopolysaccharide biosynthesis and virulence in Erwinia stewartii.},
  author={P J Dolph and Doris R. Majerczak and David L. Coplin},
  journal={Journal of bacteriology},
  year={1988},
  volume={170 2},
  pages={
          865-71
        }
}
We have previously cloned the genes for synthesis of capsular polysaccharide (cps) and slime from Erwinia stewartii in cosmid pES2144. In this study, pES2144 was shown to complement 14 spontaneous cps mutants. These mutants were characterized by probing Southern blots of mutant genomic DNA with pES2144; insertions were detected in four mutants and deletions in six mutants. Genetic and physical maps of the pES2144 cps region were constructed by subcloning, restriction analysis, and transposon… CONTINUE READING

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