Characterization of a doubly mutant derivative of the lambda PRM promoter. Effects of mutations on activation of PRM.

@article{Hwang1988CharacterizationOA,
  title={Characterization of a doubly mutant derivative of the lambda PRM promoter. Effects of mutations on activation of PRM.},
  author={Jing Jang Hwang and Spencer Brown and Gary N. Gussin},
  journal={Journal of molecular biology},
  year={1988},
  volume={200 4},
  pages={
          695-708
        }
}
The mutation, prmE37, located at -14 in the PRM promoter of bacteriophage lambda, reduces PRM function dramatically both in vitro and in vivo. In a search for second-site revertants of prmE37, we isolated a double mutant that exhibits a partially restored Prm+ phenotype. The second-site mutation (at -31) is identical to the mutation prmup-1. The activity of the doubly mutant (pseudo-revertant) promoter, prmE37prmup-1, was investigated in vivo using a PRM-lacZ fusion phage and found to be… CONTINUE READING