Characterization of a dioxygenase from Trigonella foenum-graecum involved in 4-hydroxyisoleucine biosynthesis.


A study was made of the enzyme in fenugreek implicated in the biosynthesis of 4-hydroxyisoleucine, which is an unusual amino acid known for its insulin stimulating effect. 4-Hydroxyisoleucine was detected by HPLC following isoleucine incubation with a cell-free extract from etiolated 6-day-old fenugreek seedlings in the presence of various cofactors. The… (More)


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